Year: 2013
Type: dissertation
Abstract: Stroke is the fourth leading cause of death worldwide. Low levels of IGF-1, a known neuroprotectant, correspond to aging and increased severity of stroke. We have previously demonstrated that there is an inverse relationship between the presence of anti-let7f or anti-miR-1 and levels of IGF-1. Further, the localization of let-7f was found to be within the microglial cell population. We hypothesize that let7f and miR-1 directly bind to the 3’ UTR region of IGF-1. Microglial cells (EOC-20) will be transfected with an IGF-1 clone with a luciferase tag. IGF-1 synthesis following treatment with antagomirs to either miR-1 or let7f will be tested by a luciferase assay.
Author: Kayla McEnery
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2630258023