Work

Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Year: 2002

Type: article

Abstract: A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (< 3 mum) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that lambda-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.

Source: Infoscience (Ecole Polytechnique Fédérale de Lausanne)

Authors: Thanh Tu Duong, G M Kim, Robert Ros, Martin Streek, Friederike Schmid, Juergen Brügger, Dario Anselmetti, Alexandra Ros

Institutions: Bielefeld University, Kyungpook National University, École Polytechnique Fédérale de Lausanne

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2671837978

FWCI:

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Cited by: 1

Related to: 10