Single cell analysis of drug responsive T cells; identification of candidate drug reactive T cell receptors in abacavir and carbamazepine hypersensitivity
Year: 2016
Type: conference-paper
Abstract: MethodsWorking from a cohort of cryopreserved PBMCs on HLA-typed patients with confirmed histories of T-cell mediated drug hypersensitivities (ABC HSR and CBZ induced SJS/TEN and DRESS) we performed flow cytometry, single cell cloning and single cell TCR sequencing and used these to develop and interrogate T-cell responses (Figure 2). ConclusionsSeveral candidate pathogenic TCRs have been identified in patients with hypersensitivity to abacavir or carbamazepine.These putative TCRs will be cloned into Jurkat T-cell lines to determine if multiple T-cell clones contribute to hypersensitivity or if only a single clone in each patient is pathogenic.Ultimately these cloned TCR will be used to define the crystal structure of the trimolecular complex of drug/MHC/TCR.
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2684350234