Characterization of the Mobility of FcγRIIa in Primary Human Macrophages
Year: 2010
Type: dissertation
Abstract: Fcγ receptor-mediated phagocytosis is an active process requiring receptor clustering as a signal initiation event. The mechanisms controlling Fcγ receptor clustering are unknown, as are the parameters governing the receptor lateral mobility in the plasma membrane. This work investigated the lateral mobility of Fcγ receptor IIa in resting primary human macrophages using single-molecule tracking methodology. In the absence of ligands, the receptor was found to exist mostly as a monomeric species. Detailed receptor dynamics revealed the existence of two receptor populations: one that was mobile, the other confined. The actin cytoskeleton was shown to be important for receptor confinement but did not affect receptor diffusion. Such findings are\nimportant in understanding the mechanisms for receptor clustering and signal initiation in phagocytosis.
Author: Yoav Farkash
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2684401989