Year: 2017
Type: dissertation
Abstract: Studies into muscle diseases have focussed more on the pathologies presented in mature skeletal muscle than on how this affects myogenic progenitors. Embryonic myogenesis and disease studies also rely on animal models, because of difficulty in obtaining human tissue samples. This study tested whether a step-wise approach mimicking myogenesis could derive embryonic skeletal muscle progenitors from human pluripotent stem cells (hPSC), which in other studies was limited by poor purity and disruptive genetic modification. hPSCs were differentiated to express paraxial mesoderm markers, and modulating myogenic signalling pathways enriched for cells expressing early myogenic marker Pax3. However, further myogenic differentiation in this protocol was absent. Using a recently published protocol instead for skeletal muscle derivation from hPSCs generated considerable numbers of myogenic progenitors and myocytes. However, final differentiation purity was impeded by contamination arising at the start of differentiation. This approach highlighted challenges still faced in robustly recapitulating myogenesis in vitro.
Source: TSpace (University of Toronto)
Author: James Brendan Morrissey-Scoot
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2724848570