(E)-α-bisabolene production in Synechocystis sp. PCC 6803
Year: 2017
Type: dissertation
Abstract: The world is currently facing many environmental challenges related to fossil fuels and meanwhile the global energy demand is increasing rapidly. The terpenoid (E)-α-bisabolene is a precursor of the potential diesel fuel replacement bisabolane, and has been proven possible to produce in the cyanobacterial strain Synechocystis sp. PCC 6803. The aim of this project was to increase the bisabolene production in said strain, as well as introducing the same engineered system into Synechococcus elongatus PCC 7942 for comparison. For this purpose, several constructs were constructed which all contained the first enzyme of the terpenoid biosynthesis pathway; 1-Deoxy-D-xylulose 5-phosphate synthase (DXS). These constructs were then conjugated into the cyanobacterial strains after which the bisabolene production of positive colonies was evaluated. One of the constructs proved to increase the production of bisabolene in Synechocystis sp. PCC 6803, however the introduced DXS gene could not be detected neither on a genetic nor a translational level. Furthermore, the conjugation of Synechococcus elongatus PCC 7942 proved unsuccessful and did not give any colonies.
Author: Sara C. Nilsson
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2725703522