Characterisation of chlorinated fatty acid metabolites in human cells after uptake of dichlorostearic acid, as determined by a halogenspecific detector (XSD)
Year: 2000
Type: dissertation
Abstract: Chlorinated fatty acids (CIFAs) have been found to account for a considerable portion of the \nextractable organically bo und chlorine in aquatic animals. Methods particularly suitable for the \ndetermination of CIFAs have relatively newly been developed, which may explain why these \ncompounds did not come to attention as a potentially hazardous environmental pollutant until during \nthe recent decade. The presence of CIFAs in human tissues has not yet been reported, and it is not \nknown if human cells can incorporate and metabolise these compounds. \n \nRecently a new halogen specific detector (XSD) has been introduced. It is described to be a \nsensitive, selective, and robust detector in the determination of halogenated pesticides. It has been \ndeclared to be more stable and easier to maintain than other halogen selective detectors. The XSD \noperates in an oxidative pyrolysis mode and the sample compounds are converted into their \noxidation products. When halogen-containing compounds enter the hot detector, the detector current \nwill increase. CIF As have not earlier been studied by an XSD. \n \nThe aim of this work was to study i) the XSD, connected to a gas chromatograph, in the \ndetermination of CIFA methyl esters (CIFAMEs), and ii) the incorporation and metabolism of \nCIFAs in human cell lines, as determined by XSD detection. \n \nIt was shown that the XSD provides a detection limit (0.2 ng of dichlorostearic acid methyl \nester) and a selectivity ((response CIFAMEs)/(response unchlorinated fally acid methyl esters)) >> \n10⁴ similar to that of the electrolytic conductivity detector (ELCD), commonly used in \ndetermination of CIFAMEs. Furthermore, the XSD was found to be very easy and stable to maintain \nin the analysis of CIFAMEs. \n \nIn the determination of CIFAs obtained from human cell lines, it was established that human \ncells can incorporate dichlorostearic acid and degrade it to dichloropalmitic acid and \ndichloromyristic acid, probably through α3-oxidation. CIFAs were found both in the neutrallipid and \nin the phospholipid fraction of the cultured cells. Dichloromyristic acid was the shortest CIFAs \ndetected and was found to be released to the culture medium to a higher extent than the other \nCIFAs. \n \nThe XSD was found to be a good alternative to the ELCD in the determination of CIFAs and the \nXSD is suitable for use in continued studies of the turnover of CIFAs in human cells.
Author: Gunilla Åkesson‐Nilsson
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2725922803