Work

Multisite Electrophysiology Recordings in Mice to Study Cross‐Regional Communication During Anxiety

Year: 2017

Type: article

Abstract: Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety-related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input-specific and cell-specific contributions to circuit-wide activity. We discuss the planning, execution, and troubleshooting of physiology experiments during anxiety-like behavior. © 2017 by John Wiley & Sons, Inc.

Source: Current Protocols in Neuroscience

DOI: 10.1002/cpns.32

Authors: Alexander Z. Harris, Danielle Golder, Ekaterina Likhtik

Institutions: Columbia University Irving Medical Center, Hunter College, The Graduate Center, CUNY, City University of New York

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Mental Health