Work

Irreversible Protein Labeling by Paal–Knorr Conjugation

Year: 2017

Type: article

Abstract: The application of new chemical reactions in a biological context has advanced bioconjugation methods for both fundamental research and commercial arenas. Recent adaptations of reactions such as Huisgen 1,3-dipolar or Diels-Alder cycloadditions have enabled the labeling of specific residues in biomolecules by the attachment of molecules carrying azides, alkynes, or strained alkenes. Although these are fundamental tools, there is a need for the discovery of reactions that can label native proteins. We report herein the adaptation of the Paal-Knorr reaction to label lysine residues in proteins via pyrrole linkages.

Source: ChemBioChem

DOI: 10.1002/cbic.201700210

Authors: Ramesh Dasari, James J. La Clair, Alexander Kornienko

Institutions: Texas State University, University of California San Diego, Xenobe Research Institute

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: Mode of Action of the Amaryllidaceae Alkaloid Lycorine-Promising Anticancer Agent, G8906573206