Work

Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Year: 2017

Type: article

Abstract: Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.

Source: Scientific Reports

DOI: 10.1038/s41598-017-06174-3

Authors: Stefan Lutzmayer, Balaji Enugutti, Michael D. Nodine

Institutions: Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna Biocenter

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: European Commission, Austrian Science Fund, H2020 European Research Council