Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature
Year: 2017
Type: article
Abstract: Significance A fundamental bioenergetics question is how the redox energy from oxygen reduction in cytochrome c oxidase (C c O) is coupled to proton translocation. Although crystallography has revealed many properties of the enzyme, the coupling mechanism remains elusive, in part due to artifacts caused by X-ray damage from synchrotron radiation. We determined the structure of CO-bound bovine C c O by serial femtosecond X-ray crystallography (SFX) with an X-ray free electron laser, outrunning radiation damage. The SFX structure is compared with the structure obtained with synchrotron radiation in which the Fe–CO bond was cleaved. Our results reveal that ligand binding to heme a 3 triggers an allosteric structural transition that may play a critical role in proton translocation in the C c O family of enzymes.
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: bronze
Funders: National Science Foundation, U.S. Department of Energy, Arizona State University, National Institutes of Health, Office of Science, National Institute of General Medical Sciences, National Center for Research Resources, Division of Chemistry, Division of Biological Infrastructure, Basic Energy Sciences, SLAC National Accelerator Laboratory
Awards: G1229526393, G1286251239, Mechanisms of Energy Transduction in Heme-Copper Oxidases, G2371548206, G262532246, G303061748, G4235786860, A Synchroton Radiation Structural Biology Resource, G495301720, G4978942704, G498139845, G5570207115, Dynamics of membrane proteins unraveled by time-resolved serial crystallography, G5637119208, G6550344761, Structure and Function of Heme-based Dioxygenases, G7283024673, G7288961653, ABI Innovation: New Algorithms for Biological X-ray Free Electron Laser Data, G8382000481, Reactive Intermediates in cytochrome oxidase, A Synchrotron Radiation Structural Biology Resource, Structures of intermediates in cytochrome c oxidase, G911061179, Biology with X-ray Lasers, G969889393
OpenAlex ID: W2735511747