Work

Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

Year: 2017

Type: article

Abstract: Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Source: Nature Communications

DOI: 10.1038/ncomms15993

Authors: Stefanie L. Morgan, Natasha C. Mariano, Abel Bermudez, Nicole L. Arruda, Fangting Wu, Yunhai Luo, Gautam Shankar, Lin Jia, Huiling Chen, Ji-Fan Hu, Andrew R. Hoffman, Chiao-Chain Huang, Sharon J. Pitteri, Kevin C. Wang

Institutions: Stanford Medicine, Stanford University, Bridgewater State University, System Biosciences (United States), Jilin University, VA Palo Alto Health Care System, First Hospital of Jilin University, Central South University, Xiangya Hospital Central South University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $6,990

Funders: National Science Foundation, U.S. Department of Veterans Affairs, Burroughs Wellcome Fund, Donald E. and Delia B. Baxter Foundation, National Defense Science and Engineering Graduate

Award: G7214886494

OpenAlex ID: W2736242246

FWCI: 11.65

Cites: 18

Cited by: 278

Related to: 10