Direct In Vitro Regeneration of Fig (Ficus carica L. CV. Benaty) from Apical and Lateral Buds
Year: 2016
Type: article
Abstract: Benaty fig plant was successfully propagated by tissue culture technique and areproducible and reliable micropropagation protocol was developed through thisinvestigation. At initiation stage, healthy aseptic cultures from lateral and terminal budexplants of about 85% were achieved when the explants were treated with 0.15% HgCl 2for 7 minutes. A combined solution of ascorbic acid and citric acid (100 and 150 mgl -1 )was successfully used to overcome the problem of phenolic compounds exudation fromthe explants into the culture media. At shoot multiplication stage, the addition of 2.0 mgl -1 from both BA and kinetin gave the highest number of shoots per explant estimated at2.66 and 3.33 shoots/ explant respectively. As well as, activated charcoal promotedshoots multiplication in all multiplication parameters. The highest number of shoots perexplants (5 shoots/ explant) was obtained on MS medium containing 4.0 gl -1 AC. At rootformation stage, the interaction treatment of full MS salt strength with the use of 0.25mgl -1 NAA gave the highest rooting percentage reaching 87.5%. The highest number ofroots (18.5 roots/ explant) was recorded as well from the combined treatment of full MSsalt strength with the use of 0.25 mgl -1 NAA. While the longest roots (6.66 cm) wererecorded from the interaction treatment of half salt strength and 0.50 mgl -1 IBA. Theaddition of activated charcoal to the rooting culture medium decreased the rootingpercentage from 87.5% at control treatment to 50% and 62.5% when 2.0 and 4.0 gl -1 ACwere respectively added. The in vitro propagated fig plantlets were gradually moved in asuccessful way from lab to field conditions with 95% survival rate.
DOI: 10.17656/jzs.10453
Authors: Rafail Toma, Yousif Tamer
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2737057797