Year: 2018
Type: article
Abstract: Biofilms are communities of bacteria that exhibit a multitude of multiscale biomechanical behaviours. Recent experimental advances have led to characterisations of these behaviours in terms of measurements of the viscoelastic moduli of biofilms grown in bioreactors and the fracture and fragmentation properties of biofilms. These properties are macroscale features of biofilms; however, a previous work by our group has shown that heterogeneous microscale features are critical in predicting biofilm rheology. In this paper, we use tools from statistical physics to develop a generative statistical model of the positions of bacteria in biofilms. Specifically, the model is a type of pairwise interaction model (PIM). We show through simulation that the macroscopic mechanical properties of biofilms depend on the choice of microscale spatial model. A key finding is that uniform and non-uniform sets of points lead to differing mechanical properties. This distinction appears not to have been previously considered in mathematical biofilm literature. We also found that realisations of a biologically informed PIM have realisticin silicomechanical properties, and have statistical properties that closely match experimental data. We also note that a Poisson spatial point process of suitable number density also yields realistic mechanical properties, but that the spatial distribution of points does not reflect those occurring in our experimentally observed biofilm.
Institution: University of Colorado Boulder
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G1314282440, G1887789060, G222768804, A GRADUATE FELLOWSHIP PROGRAM IN COMPUTATIONAL SCIENCE AND RELATED SCIENCES, Collaborative Research: Type II: Flow-induced fragmentation mechanisms in bacterial biofilms by hierarchical modeling of polymeric, interfacial and viscoelastic interactions, Collaborative Research: Microbial Flocculation Dynamics, G968891095
OpenAlex ID: W2737922484