MOESM2 of Authentication of collagen VI antibodies
Year: 2017
Type: other
Abstract: Additional file 2: Figure S2. Experimental controls for immunohistochemical and immunoblot experiments. Normal human skeletal muscle cross- and transverse sections were stained for rabbit (panels A and B), mouse (C and D) and goat (E and F) IgGs were used at a dilution of 1 in 100 and detected using the indicated fluorescently-labelled secondary antibodies. All controls were blank. Blots containing lysates from HEK-293 cells transfected with all three chains were probed for mouse IgG (panel G), normal rabbit (H) or normal goat (I) serum. Migration positions of molecular weight markers are shown on left. A faint band at 70-75 kDa is present in panel H (indicated by asterisk).
Source: Figshare
Authors: Endicott, Jamie, Holden, Paul, Fitzgerald, Jamie
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2739854182