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Preparation of Mosquito Salivary Gland Extract and Intradermal Inoculation of Mice

Year: 2017
Type: article
Abstract: parasites, among others. Here, we describe standardized methods to assess the impact of mosquito-derived factors on immune responses and pathogenesis in mouse models of infection. This protocol includes the generation of mosquito salivary gland extracts and intradermal inoculation of mouse ears. Ultimately, the information obtained from using these techniques can help reveal fundamental mechanisms of interaction between pathogens, mosquito vectors, and the mammalian host. In addition, this protocol can help establish improved infection challenge models for pre-clinical testing of vaccines or therapeutics that take into account the natural route of transmission via mosquitoes.
Source: BIO-PROTOCOL
DOI: 10.21769/bioprotoc.2407
Institutions: Rega Institute for Medical Research, KU Leuven, New York State Department of Health, Wadsworth Center, University of California, Berkeley, State University of New York, University at Albany, State University of New York
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2742284448
FWCI: 0.767
Cites: 15
Cited by: 28
Related to: 10