Year: 2017
Type: review
Abstract: Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).
Authors: Nathalie Percie du Sert, Alessio Alfieri, Stuart M Allan, Hilary VO Carswell, Graeme A Deuchar, Tracy D Farr, Paul Flecknell, Lindsay Gallagher, Claire L Gibson, Michael J Haley, Malcolm R Macleod, Barry W McColl, Christopher McCabe, Anna Morancho, Lawrence DF Moon, Michael J O’Neill, Isabel Pérez de Puig, Anna Planas, C Ian Ragan, Anna Rosell, Lisa A Roy, Kathryn O Ryder, Alba Simats, Emily S Sena, Brad A Sutherland, Mark D Tricklebank, Rebecca C Trueman, Lucy Whitfield, Raymond Wong, I Mhairi Macrae
Institutions: National Centre for the Replacement Refinement and Reduction of Animals in Research, Roslin Institute, University of Edinburgh, University of Manchester, University of Strathclyde, University of Glasgow, University of Nottingham, Newcastle University, University of Leicester, Universitat Autònoma de Barcelona, Vall d'Hebron Institut de Recerca, King's College London, Eli Lilly (United Kingdom), Consejo Superior de Investigaciones Científicas, Institut d'Investigacions Biomèdiques de Barcelona, Consorci Institut D'Investigacions Biomediques August Pi I Sunyer, Home Office, University of Tasmania, University of Oxford, Royal Veterinary College
Language: English
Subfield: Small Animals
Field: Veterinary
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,200
Awards: G1551429087, Complex phenotypes and genotype x environment interactions, Identifying mechanisms regulating central nervous system homeostasis, Role of peripheral interleukin-1 in cerebrovascular disease, TREM-2, inflammation resolution and the transition from brain injury to repair after stroke, G7517174637
OpenAlex ID: W2742992776