Work

Cloning, optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli.

Year: 2017

Type: article

Abstract: BACKGROUND AND OBJECTIVES: for purification. MATERIALS AND METHODS: BL21 (DE3) cells. To evaluate the induction conditions for optimized expression, factorial design of experiments was employed using four different media as well as four levels of isopropyl-b-D-thiogalactopyranosid [IPTG] concentration and duration of induction. The recombinant protein was then purified using a His-tag purification kit and detected through western blotting. RESULTS: which was successfuly purified. CONCLUSION: might be a good candidate for vaccine production against tuberculosis.

Source: PubMed

Authors: Mitra Ashayeri-Panah, Fereshteh Eftekhar, Bahram Kazemi, Joan Joseph

Institutions: Shahid Beheshti University, Shahid Beheshti University of Medical Sciences, Universitat de Barcelona

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2745373761

FWCI: 0.195

Cites: 24

Cited by: 5

Related to: 10