Work

Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy

Year: 2017

Type: article

Abstract: We introduce a concept that enables parallelized three-dimensional imaging throughout large volumes with isotropic 300-350 nm resolution. By staggering high aspect ratio illumination beams laterally and axially within the depth of focus of a digitally scanned light-sheet fluorescence microscope (LSFM), multiple image planes can be simultaneously imaged with minimal cross-talk and light loss. We present a first demonstration of this concept for parallelized imaging by synthesizing two light-sheets with nonlinear Bessel beams and perform volumetric imaging of fluorescent beads and invasive breast cancer cells. This work demonstrates that in principle any digitally scanned LSFM can be parallelized in a lossless manner, enabling drastically faster volumetric image acquisition rates for a given sample brightness and detector technology.

Source: Scientific Reports

DOI: 10.1038/s41598-017-08113-8

Authors: Kevin M. Dean, Reto Fiolka

Institution: The University of Texas Southwestern Medical Center

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: Cancer Prevention and Research Institute of Texas, National Institutes of Health, National Institute of General Medical Sciences