Year: 2017
Type: article
Abstract: The present study was designed to study the presence of intimin gene and its variants in 91 E. coli isolates. All 91 E. coli isolates were screened for the presence of intimin gene by PCR, among which only 15 isolate (16.8 percent) were positive for eaeA gene. Out of 15 isolates positive for eae (intimin) were assayed for intimin variants viz., eae1(811bp), eae1(804bp), eae2(808bp), eae (833bp), eae (206bp), and eae (468bp) by PCR. Eight isolates could be typed using 6 intimin variant primer set and 2 isolate (13.3percent) were positive for eae1 variant, one isolate (6.67percent) was positive for eae1 and five (33.3percent) were positive for eae2. Intimin gene codes for different types of protein which are immunogenic in nature and thus it can represent an important tool for E. coli typing in routine diagnostic as well as epidemiological and clonal studies. Different intimin variants are responsible for different host tissue cell tropism which could be taken into consideration to identify the specific intimin variant types responsible for attachment to specific tissue tropism.
Authors: Vandana Gupta, Ashish Roy, Megha Katare
Institution: Awadhesh Pratap Singh University
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2746862497