Year: 2012
Type: article
Abstract: We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.
Source: Journal of Biomedical Optics
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funders: National Research Foundation, Ministry of Education, Science and Technology, Korea Advanced Institute of Science and Technology, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources
Awards: Quantitative Analysis of Blood Flow in Sickle Cell Disease, MIT LASER BIOMEDICAL RESEARCH CENTER, G2966330512, G3000594302, G3430448037, G5574303639, G5594481954, G6307668300, Multiscale Modeling and Parallel Simulations of Blood Flow in Cerebral Malaria an, G7969377991, G8116902425, G9000145732, G9000192120
OpenAlex ID: W2018357067