Helicobacter pylori sabA gene is associated with iron deficiency anemia in childhood and adolescence
Year: 2017
Type: article
Abstract: BACKGROUND: Gastric Helicobacter pylori colonization leads to iron deficiency anemia (IDA), especially in children and adolescents. However the pathogenesis is poorly understood. OBJECTIVE: We sought to identify specific H. pylori genes involved in IDA development, by comparing bacterial genome-wide expression profiling in patients affected or not. METHODS: H. pylori were isolated from four children with IDA and four from matched controls without IDA. Based on these isolates, cDNA microarrays under iron-replete or depleted conditions were systematically performed to compare gene expression profiles at the whole genome level. Real-time reverse-transcription (RT-) PCR and protein assays were performed for further assessing the profile differentiation of the identified H. pylori IDA-associated genes. RESULTS: We identified 29 and 11 genes with significantly higher or lower expression in the IDA isolates compared to non-IDA isolates, respectively. Especially notable were higher expression of sabA gene encoding sialic acid-binding adhesin in the IDA isolates, which was confirmed by real-time RT-PCR study. Moreover, iron-depletion in vitro led to up-regulation of fecA1 and frpB1 genes and down-regulation of pfr, as predicted. Known iron-regulated genes such as fur, pfr, fecA, and feoB did not significantly differ between both groups. The IDA isolates had significantly higher expression of vacuolating cytotoxin gene vacA than non-IDA isolates, consistent with the results of VacA protein assays. There were no significant differences in bacterial growth value between IDA and non-IDA isolates. CONCLUSIONS: It is likely that H. pylori carrying high expression of sabA causes IDA, especially in children and adolescents who have increased daily iron demand. In addition, it is possible that several host-interactive genes, including vacA, may play a synergistic role for sabA in IDA development.
Source: PLoS ONE
Institutions: Kyorin University, Tohoku University, New York University
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1406054181, Omics analysis for elucidation of pathogenic bacterial clearance mechanism of resident bacterial flora, Study of Helicobacter pylori genes associated with iron deficiency anemia using DNA microarray, Role of stringent response in survival, colonization and expression of pathogenesis in Helicobacter pylori, Mathematical Models of H. Pylori gastric colonization, Elucidation of the pathological factor of Helicobacter pylori isolated from iron deficient anemia patient, Search for H. pylori genes and epigenetic status by Pacbio decoding
OpenAlex ID: W2751406145