Year: 2017
Type: review
Abstract: Precise manipulation of human cell fate is a long-standing goal in biological engineering; it underpins efforts to advance new cellular therapeutics, and to develop disease models for fundamental research. Human pluripotent stem cells (hPSC) are emerging as the cells of choice for many of these applications. Currently, most advanced methods for manipulation of hPSC fate involve recapitulating developmental processes by mimicking stem cell niche-associated signals. Alternately, advances in engineering synthetic decision-making gene circuits provide an approach in which cell fate is directly controlled at the gene expression level. Here, we make the case that integrating these two engineering approaches represents an exciting opportunity to advance our fundamental understanding of cell fate control, and to accelerate new engineering strategies to manipulate cellular behavior for therapy.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,170
Funders: National Science Foundation, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Eidgenössische Technische Hochschule Zürich, University of Toronto, Canada First Research Excellence Fund, Canadian Institutes of Health Research, European Research Council, National Center of Competence in Research Quantum Science and Technology, H2020 European Research Council
OpenAlex ID: W2756796266