Year: 2011
Type: dissertation
Abstract: As a consequence of their extensive use in the last decades more and more classical antibiotics lose their activity against emerging multi-resistant microorganisms. Antimicrobial peptides (AMPs) are thought to be an attractive alternative to convential antibiotics. But the synthetic production of these peptides is very expensive. Hence, the economic production in different Nicotiana species serving as inexpensive green factories was evaluated during this study. As method of choice for in planta peptide production a Tobacco mosaic virus (TMV) derived expression system with the viral coat protein as fusion partner was generated. Following successful production of the desired peptides in Nicotiana benthamiana, an efficient purification protocol was elaborated. The final yield ranged between 0.2 and 0.5 mg of pure peptide per 20 g of infected fresh leaf biomass and in vitro antimicrobial activity was successfully proved.
Source: Site cant be reached
Author: Benjamin Zeitler
Language: English
Subfield: Biotechnology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2756987038