Work

Scanning Electron Microscopy of Motile Male Gametes of Land Plants

Year: 2017

Type: article

Abstract: and cycads. This wide diversity in number of flagella is associated with vast differences in cell size and shape. Scanning electron microscopy (SEM) has played an important role in characterizing the external form, including cell shape and arrangement of flagella, across the varied motile gametes of land plants. Because of the size and scarcity of released swimming sperm, it is difficult to concentrate them and prepare them for observation in the SEM. Here we detail an SEM preparation technique that yields good preservation of sperms cells across plant groups.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2570

Authors: Karen Renzaglia, Renee Lopez, Steven Schmitt

Institution: Southern Illinois University Carbondale

Language: English

Topic: Plant Reproductive Biology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Science Foundation

Awards: ATOL: Collaborative Research: Deep Green Plant Phylogenetics: Novel Analytical Methods for Scaling Data from Genomics to Morphology, Acquisition of an Electron Microscope with Digital Camera and Ultramicrotomes for a Central Research Facility