Characterization and Validation of a Novel Group of Type V, Class 2 Nucleases for in vivo Genome Editing
Year: 2017
Type: preprint
Abstract: CRISPR-based genome editing is an enabling technology with potential to dramatically transform multiple industries. Identification of additional editing tools will be imperative for broad adoption and application of this technology. A novel Type V, Class 2 CRISPR nuclease system was identified from Microgenomates and Smithella bacterial species ( C RISPR from M icrogenomates and S mithella , Cms1). This system was shown to efficiently generate indel mutations in the major crop plant rice ( Oryza sativa ). Cms1 are distinct from other Type V nucleases, are smaller than most other CRISPR nucleases, do not require a tracrRNA, and have an AT-rich protospacer-adjacent motif site requirement. A total of four novel Cms1 nucleases across multiple bacterial species were shown to be functional in a eukaryotic system. This is a major expansion of the Type V CRISPR effector protein toolbox and increases the diversity of options available to researchers.
DOI: 10.1101/192799
Authors: Matthew B. Begemann, Benjamin N. Gray, Emma January, Anna Singer, Dylan C. Kesler, Yonghua He, Haijun Liu, Hongjie Guo, Alex Jordan, Thomas P. Brutnell, Todd C. Mockler, Mohammed Oufattole
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2757632346