Year: 2017
Type: preprint
Abstract: The Illumina HiSeq X platform has helped to reduce the cost of whole genome sequencing substantially, but its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and the elimination of a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
DOI: 10.1101/193193
Authors: M. Suzuki, W. Liao, F. Wos, A.D. Johnston, J. DeGrazia, J. Ishii, T. Bloom, M.C. Zody, S. Germer, J.M. Greally
Institutions: Albert Einstein College of Medicine, New York Genome Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: New York Genome Center
OpenAlex ID: W2758258560