Work

Purification of Native Arp2/3 Complex from Bovine Thymus

Year: 2013

Type: book-chapter

Abstract: The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-538-5_14

Authors: Lynda K. Doolittle, Michael K. Rosen, Shae B. Padrick

Institutions: Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences

Award: Structural Study of GTPase Regulators and Effectors