Immortalization of MIP-GFP Neurons from the Hypothalamus and Neuronal Cell Types from Human Islet Isolates
Year: 2013
Type: dissertation
Abstract: The mouse insulin I promoter construct was developed to eliminate the unintended hypothalamic activity seen in the rat insulin II promoter. Given the importance of the hypothalamus in the maintenance of energy homeostasis, the specificity of this construct must be validated. To investigate the possible promoter activity within the hypothalamus, primary hypothalamic cultures of fluorescent reporter mice were immortalized and sorted. The resulting mHypoA-MIP/GFP cell lines confirmed the activity of the promoter within hypothalamic neurons and act as representative neuronal models. Initial characterization has determined the insulin responsive nature of these NPY neurons. Limited in vivo validations have confirmed the NPY nature of these neurons but were unable to pinpoint their localization within the hypothalamus. Subsequently, the technology utilized was adapted to attempt to generate immortalized human islet cells with limited success. The newly generated cell models can be utilized to refine the promoter construct and human islet immortalization process.
Source: TSpace (University of Toronto)
Author: Zi Chen Wang
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2759675892