Distinct Effects of Ca2+ Sparks on Cerebral Artery and Airway Smooth Muscle Cell Tone in Mice and Humans
Year: 2017
Type: article
Abstract: The effects of Ca 2+ sparks on cerebral artery smooth muscle cells (CASMCs) and airway smooth muscle cells (ASMCs) tone, as well as the underlying mechanisms, are not clear.In this investigation, we elucidated the underlying mechanisms of the distinct effects of Ca 2+ sparks on cerebral artery smooth muscle cells (CASMCs) and airway smooth muscle cells (ASMCs) tone.In CASMCs, owing to the functional loss of Ca 2+ -activated Cl -(Clca) channels, Ca 2+ sparks activated large-conductance Ca 2+ -activated K + channels (BKs), resulting in a decreases in tone against a spontaneous depolarization-caused high tone in the resting state.In ASMCs, Ca 2+ sparks induced relaxation through BKs and contraction via Clca channels.However, the integrated result was contraction because Ca 2+ sparks activated BKs prior to Clca channels and Clca channels-induced depolarization was larger than BKs-caused hyperpolarization.However, the effects of Ca 2+ sparks on both cell types were determined by L-type voltage-dependent Ca 2+ channels (LVDCCs).In addition, compared with ASMCs, CASMCs had great and higher amplitude Ca 2+ sparks, a higher density of BKs, and higher Ca 2+ and voltage sensitivity of BKs.These differences enhanced the ability of Ca 2+ sparks to decrease CASMC and to increase ASMC tone.The higher Ca 2+ and voltage sensitivity of BKs in CASMCs than ASMCs were determined by the β1 subunits.Moreover, Ca 2+ sparks showed the similar effects on human CASMC and ASMC tone.In conclusions, Ca 2+ sparks decrease CASMC tone and increase ASMC tone, mediated by BKs and Clca channels, respectively, and finally determined by LVDCCs.
DOI: 10.7150/ijbs.21475
Authors: Qing-Yang Zhao, Yong-Bo Peng, Xiao-Jing Luo, Xi Luo, Hao Xu, Ming-Yu Wei, Qiu-Ju Jiang, Wen-Er Li, Li-Qun Ma, Jin-Chao Xu, Xiao-Cao Liu, Dun-An Zang, Yu-San She, He Zhu, Jinhua Shen, Ping Zhao, Lu Xue, Meng-Fei Yu, Weiwei Chen, Ping Zhang, Xiangning Fu, Jingyu Chen, Xiaowei Nie, Chenyou Shen, Shu Chen, Shanshan Chen, Jingcao Chen, Sheng Hu, Chunbin Zou, Gangjian Qin, Ying Fang, Jiuping Ding, Guangju Ji, Yun-Min Zheng, Tengyao Song, Yong-Xiao Wang, Qing-Hua Liu
Institutions: South Central Minzu University, Technische Universität Dresden, Tongji Hospital, Huazhong University of Science and Technology, Wuhan University, Zhongnan Hospital of Wuhan University, Wuxi People's Hospital, Nanjing Medical University, Wuhan Union Hospital, Hubei Cancer Hospital, University of Pittsburgh, University of Alabama, University of Alabama at Birmingham, Chinese Academy of Sciences, Institute of Biophysics, Albany Medical College
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,337
Awards: Signaling Mechanisms for Hypoxic Pulmonary Vasoconstriction, 猪JHDM1A基因及其调节的组蛋白H3K36去甲基化对肌发生的调控作用研究, G2568343366, Epigenetic Regulation in Acute Lung Injury, G3115193368, G6519738610, G7386149582, Novel signaling in chronic hypoxic responses in pulmonary arteries, G8768935877, 组蛋白去甲基化酶FBXL11基因对哮喘ASMC增殖和功能的调控作用及分子机制
OpenAlex ID: W2759686293