Work

A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech

Year: 2017

Type: article

Abstract: Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging, we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.

Source: eLife

DOI: 10.7554/elife.29839

Authors: Yusuke Tomina, Daniel A Wagenaar

Institution: California Institute of Technology

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: Burroughs Wellcome Fund, Japan Society for the Promotion of Science, National Institute of Neurological Disorders and Stroke