The Expression Profile of KIAA0319-like in Chick Embryos and its Involvement in Cell Migration in the Developing Optic Tectum
Year: 2011
Type: dissertation
Abstract: Several genes thought to confer susceptibility to dyslexia have been identified, and the purpose of this study is to 1) determine the expression pattern of one of these gene products and 2) characterize the function of the product of one of these genes, namely KIAA0319-Like (KIAA0319L), using the developing chick visual system as a\nmodel.\nWhole mount in situ hybridization was performed for KIAA0319L on embryonic\nday (E)3 – E5 and in situ hybridization on sections was performed at later stages.\nEngineered microRNA (miRNA) constructs targeting KIAA0319L were prepared and\ntheir specificity and efficiency for knocking down KIAA0319L were tested. miRNAs\nwere electroporated in E5 optic tecta (OT). Embryos were sacrificed at E12. OT were\nremoved, sectioned and analyzed.\nResults demonstrate that KIAA0319L is expressed in the developing chick visual\nsystem. Knockdown of KIAA0319L in the OT results in abnormal migration indicating\nthat KIAA0319L is necessary for this process.
Source: TSpace (University of Toronto)
Author: Jason Charish
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2761772449