Identifying Host Factors Associated with DNA Replicated During Virus Infection
Year: 2017
Type: article
Abstract: Viral DNA genomes replicating in cells encounter a myriad of host factors that facilitate or hinder viral replication. Viral proteins expressed early during infection modulate host factors interacting with viral genomes, recruiting proteins to promote viral replication, and limiting access to antiviral repressors. Although some host factors manipulated by viruses have been identified, we have limited knowledge of pathways exploited during infection and how these differ between viruses. To identify cellular processes manipulated during viral replication, we defined proteomes associated with viral genomes during infection with adenovirus, herpes simplex virus and vaccinia virus. We compared enrichment of host factors between virus proteomes and confirmed association with viral genomes and replication compartments. Using adenovirus as an illustrative example, we uncovered host factors deactivated by early viral proteins, and identified a subgroup of nucleolar proteins that aid virus replication. Our data sets provide valuable resources of virus-host interactions that affect proteins on viral genomes.
Source: Molecular & Cellular Proteomics
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1375395185, G1564983540, G1898711858, Training in Neurovirology, Role of Histone and Histone-like Mutations in the Oncogenesis of Human Cancers, Role of DNA damage in the early steps of HSV infection and latency in neurons, G567625970, Viral Modulation of Genetic Stability, Investigating chromatin control by an Adenovirus histone-like protein, Identifying proteins involved in virus DNA replication, G9000024894, G9000107195, G9000134799, G9000235721, Viral modulation of epitranscriptomic mechanisms, G925733875
OpenAlex ID: W2763673465