Work

In vivo–generated thrombin and plasmin do not activate the complement system in baboons

Year: 2017

Type: article

Abstract: challenge activated the complement system, reaching a maximum at 2 hours postchallenge during the peaks of lipopolysaccharide and bacteremia but not of TAT and PAP. Despite inducing a strong burst of thrombin and plasmin, FXa/PCPS infusion did not produce measurable levels of complement activation in vivo. Similarly, ex vivo incubation of baboon serum with thrombin, plasmin, or FXa did not show noticeable complement cleavage unless supraphysiologic amounts of enzymes were used. Our results suggest that in vivo-generated thrombin and plasmin do not directly activate the complement in nonhuman primates.

Source: Blood

DOI: 10.1182/blood-2017-06-788216

Authors: Ravi S. Keshari, Robert Silasi, Cristina Lupu, Fletcher B. Taylor, Florea Lupu

Institution: Oklahoma Medical Research Foundation

Language: English

Topic: Complement system in diseases

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Center for Research Resources