Work

Cloning of Nrf1, an NF-E2-related transcription factor, by genetic selection in yeast.

Year: 1993

Type: article

Abstract: We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.90.23.11371

Authors: J Y Chan, X L Han, Y W Kan

Institution: University of California, San Francisco

Language: English

Topic: Genomics, phytochemicals, and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

Award: G2527940301