Work

Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants

Year: 2017

Type: article

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated protein 9 (Cas9), including specific guide RNAs (gRNAs), can excise integrated human immunodeficiency virus type 1 (HIV-1) provirus from host chromosomes. To date, anti-HIV-1 gRNAs have been designed to account for off-target activity, however, they seldom account for genetic variation in the HIV-1 genome within and between patients, which will be crucial for therapeutic application of this technology. This analysis tests the ability of published anti-HIV-1 gRNAs to cleave publicly available patient-derived HIV-1 sequences to inform gRNA design and provides basic computational tools to researchers in the field.

Source: Scientific Reports

DOI: 10.1038/s41598-017-12612-z

Authors: Will Dampier, Neil T. Sullivan, Cheng-Han Chung, Joshua Chang Mell, Michael R. Nonnemacher, Brian Wigdahl

Institutions: Drexel University, Thomas Jefferson University, Sidney Kimmel Cancer Center

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,690