Monitoring Exercise- and Diet-Induced Changes in Tibial Blood Perfusion with Laser Doppler Flowmetry in Mice [abstract]
Year: 2016
Type: conference-paper
Abstract: Vascular function plays a critical role in bone development and repair, and exercise, which stimulates vascular growth and performance [1], may ameliorate detrimental bone changes associated with diabetes or stroke [2]. Measuring changes in bone vasculature in vivo is challenging, and current techniques to monitor functional changes in bone perfusion longitudinally are ineffective. Laser Doppler flowmetry (LDF) can quantify vascular changes in murine tibiae and provide a functional measure of bone perfusion beyond vascular density from histology or growth factor concentrations in serum [3]. However, a previous study reported local inflammation lasting several months, which could confound longitudinal flow measurements [3] and alter normal gait. We performed a less invasive LDF procedure to minimize inflammation and allow longitudinal perfusion measurements. We hypothesized our procedure would detect changes in tibial blood perfusion of young adult male mice with exercise and high fat diet-induced obesity without altering gait.
DOI: 10.17615/rsw4-7r09
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2766434247