Work

Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System

Year: 2017

Type: article

Abstract: Extracellular vesicles (EVs), including exosomes, are nanoscale membrane particles shed from cells and contain cellular proteins whose makeup could inform cancer diagnosis and treatment. Most analyses have focused on surface proteins while analysis of intravesicular proteins has been more challenging. Herein, we report an EV screening assay for both intravesicular and transmembrane proteins using a nanoplasmonic sensor. Termed iNPS (intravesicular nanoplasmonic system), this platform used nanohole-based surface plasmon resonance (SPR) for molecular detection. Specifically, we (i) established a unified assay protocol to detect intravesicular as well as transmembrane proteins; and (ii) engineered plasmonic substrates to enhance detection sensitivity. The resulting iNPS enabled sensitive (0.5 μL sample per marker) and high-throughput (a 10 × 10 array) detection for EV proteins. When applied to monitor EVs from drug-treated cancer cells, the iNPS assay revealed drug-dependent unique EV protein signatures. We envision that iNPS could be a powerful tool for comprehensive molecular screening of EVs.

Source: ACS Photonics

DOI: 10.1021/acsphotonics.7b00992

Authors: Jongmin Park, Hyungsoon Im, Seonki Hong, Cesar M. Castro, Ralph Weissleder, Hakho Lee

Institutions: Massachusetts General Hospital, Center for Systems Biology, Harvard University

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green