Work

Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells

Year: 2017

Type: article

Abstract: , using two different culture systems to assess their potential regarding proliferation and differentiation in a reductionist model. While the neurosphere assay is primarily performed to directly study the proliferative and differentiation potential of cells in individual brains, the monolayer culture allows single cell analysis in a rather homogeneous cell population. Here, we describe the isolation, culturing methods and differentiation of neural precursor cells in both systems.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2603

Authors: Stefanie Bernas, Odette Leiter, Tara Walker, Gerd Kempermann

Institutions: Center for Regenerative Therapies Dresden, German Center for Neurodegenerative Diseases (DZNE) Dresden, Technische Universität Dresden

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2767084255

FWCI: 0.617

Cites: 10

Cited by: 15

Related to: 10