Work

Sorcin induces gastric cancer cell migration and invasion contributing to STAT3 activation

Year: 2017

Type: article

Abstract: // Huan Tuo 1, * , Feng Shu 1, * , Sha She 1 , Min Yang 1 , Xiao Qin Zou 1 , Juan Huang 1 , Huai Dong Hu 1, 2 , Peng Hu 1, 2, 3 , Hong Ren 1, 2, 3 , Shi Fang Peng 4, 5 and Yi Xuan Yang 1, 2, 3 1 Department of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China 2 Institute for Viral Hepatitis of Chongqing Medical University, Chongqing 400016, China 3 Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, China 4 Department of Infectious Diseases, Xiangya Hospital, Central South University, Hunan 410008, China 5 Department of Health Management Center, Xiangya Hospital, Central South University, Hunan 410008, China * These authors have contributed equally to this work Correspondence to: Yi Xuan Yang, email: [email protected] Shi Fang Peng, email: [email protected] Keywords: gastric cancer (GC); sorcin; migration; invasion; iTRAQ Received: July 08, 2017 Accepted: September 21, 2017 Published: October 31, 2017 ABSTRACT Gastric cancer (GC) is a globally occurring malignancy that is characterized by a high mortality rate due to a high tendency to metastasize and poor prognoses. Sorcin, as known as SRI, a soluble resistance-related calcium-binding protein, plays a significant role in multidrug resistance. Sorcin is related to the migration and invasion of cancer cells. However, the mechanism remains unclear. Here, we used immunohistochemistry to confirm that the expression of sorcin in cancer tissues is higher than that in the adjacent normal tissues. The wound healing and transwell results indicate that sorcin can induce migration and invasion of GC cells. To explore the role of sorcin in GC metastasis, isobaric tags for relative and absolutely quantitation (iTRAQ) were used to examine cells with and without sorcin knockdown to identify the differentially expressed proteins (DEPs). The results were evaluated via RT-PCR and western blot to confirm the ITRAQ data. Inhibition of sorcin expression can down- regulate the expression of CTSZ, MMP2, MMP9 and p-STAT3 followed by suppression of tumor growth and metastasis. Together, we concluded that sorcin has a oncogenic activity via inducing tumor growth and metastasis, leading to development of therapeutic treatments for GC.

Source: Oncotarget

DOI: 10.18632/oncotarget.22208