A bacterial GW-effector targets Arabidopsis AGO1 to promote pathogenicity and induces Effector-triggered immunity by disrupting AGO1 homeostasis
Year: 2017
Type: preprint
Abstract: Pseudomonas syringae (P.syringae) type-III effectors were previously found to suppress the Arabidopsis microRNA (miRNA) pathway through unknown mechanisms.Here, we first show that the P. syringae HopT1-1 effector promotes pathogenicity by suppressing the Arabidopsis Argonaute 1 (AGO1)dependent miRNA pathway.We further demonstrate that HopT1-1 physically interacts with Arabidopsis AGO1 through conserved glycine/tryptophan (GW) motifs.Importantly, this AGO-binding platform was found to be essential for the ability of HopT1-1 to suppress both miRNA activity and PAMP-Triggered Immunity (PTI).These results therefore indicate that the RNA silencing suppression activity of HopT1-1 is intimately coupled to its virulence function.Overall, these findings provide the first evidence that a bacterial effector has evolved to directly target an AGO protein to suppress PTI and cause disease.
Source: HAL
DOI: 10.1101/215590
Institutions: Centre National de la Recherche Scientifique, Inserm, Université Paris Sciences et Lettres, École Normale Supérieure - PSL, Institut de Biologie de l'École Normale Supérieure, University of Nebraska–Lincoln, Université de Perpignan, Laboratoire Génome et Développement des Plantes, Laboratoire des Interactions Plantes Microbes Environnement, Université de Toulouse
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Funder: Agence Nationale de la Recherche
OpenAlex ID: W2767456780