Work

Detection of N 6 -methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

Year: 2017

Type: article

Abstract: -methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.

Source: Chemical Communications

DOI: 10.1039/c7cc07699a

Authors: Miki Imanishi, Shogo Tsuji, Akiyo Suda, Shiroh Futaki

Institutions: Kyoto University, Kyoto University Institute for Chemical Research

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Japan Society for the Promotion of Science, Core Research for Evolutional Science and Technology

Awards: G8080138678, Sequence specific detection and manipulation of epigenetic status