Work

Characterization of drug-induced splicing complexity in prostate cancer cell line using long read technology

Year: 2017

Type: conference-paper

Abstract: We characterize the transcriptional splicing landscape of a prostate cancer cell line treated with a previously identified synergistic drug combination. We use a combination of third generation long-read RNA sequencing technology and short-read RNAseq to create a high-fidelity map of expressed isoforms and fusions to quantify splicing events triggered by treatment. We find strong evidence for drug-induced, coherent splicing changes which disrupt the function of oncogenic proteins, and detect novel transcripts arising from previously unreported fusion events.

Source: Pacific Symposium on Biocomputing (PSB)

DOI: 10.1142/9789813235533_0002

Authors: Xintong Chen, Sander Houten, Kimaada Allette, Robert P. Sebra, Gustavo Stolovitzky, Bojan Losic

Institutions: Icahn School of Medicine at Mount Sinai, IBM (United States), IBM Research - Thomas J. Watson Research Center

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W2768834792

FWCI: 2.043

Cites: 28

Cited by: 5

Related to: 10