Work

In vivo bioluminescence imaging of labile iron accumulation in a murine model of Acinetobacter baumannii infection

Year: 2017

Type: article

Abstract: Significance Iron is a required metal nutrient for life, and its altered homeostasis is associated with a number of diseases. We present a bioluminescent reporter for visualizing iron pools in living animals, where iron-dependent uncaging of d -aminoluciferin enables sensitive and selective imaging of ferrous over ferric forms of iron in luciferase-expressing cell and mouse models. Application of this technology to a model of systemic bacterial infection reveals elevation of iron in infected tissues that accompany markers for increased iron acquisition and retention. These data establish the ability to assess iron status in living animals and provide a unique platform for studying its contributions to stages of health, aging, and disease.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1708747114

Authors: Allegra T. Aron, Marie C. Heffern, Zachery R. Lonergan, Mark N. Vander Wal, Brian R. Blank, Benjamin Spangler, Yaofang Zhang, Hyo Min Park, Andreas Stahl, Adam R. Renslo, Eric P. Skaar, Christopher J. Chang

Institutions: University of California, Berkeley, Vanderbilt University, Vanderbilt University Medical Center, University of California, San Francisco, Howard Hughes Medical Institute

Language: English

Topic: bioluminescence and chemiluminescence research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Environmental Health Sciences

Awards: G2725583501, University of California Berkeley Chemistry-Biology Interface Training Program, G3485139178, G464776573, Replacement and Upgrade of an Optical Imaging System for Small Animals, G540534827, Matrix assisted cell transplantation of brown fat, G6276057392, G9000049458, G9000091806, G9000297299, Molecular and Cellular Basis for Digestive Diseases, Chemical Probes to Study Copper Biology, Host-mediated zinc sequestration during Acinetobacter baumannii infection

OpenAlex ID: W2769764749

FWCI: 5.24

Cites: 78

Cited by: 132

Related to: 10