Work

Expression and purification of untagged GlnK proteins from actinobacteria

Year: 2017

Type: article

Abstract: Circular dichroism and gel filtration analysis supported that the purified proteins are correctly folded. The purification protocol described here will facilitate biochemical studies and help to uncover the biochemical functions of PII proteins in Actinobacteria.

Source: EXCLI journal

DOI: 10.17179/excli2017-394

Authors: Gerhardt, Edileusa C.M., Moure, Vivian R., Souza, Andrey W., Pedrosa, Fabio O., Souza, Emanuel M., Diacovich, Lautaro, Gramajo, Hugo, Huergo, Luciano F.

Institutions: Universidade Federal do Paraná, Consejo Nacional de Investigaciones Científicas y Técnicas, National University of Rosario, Instituto de Biología Molecular y Celular de Rosario

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação Araucária

OpenAlex ID: W2769848127

FWCI:

Cites: 23

Cited by: 1

Related to: 10