Distinct Gene Expression and Epigenetic Signatures in Hepatocyte-like Cells Produced by Different Strategies from the Same Donor
Year: 2017
Type: article
Abstract: Hepatocyte-like cells (HLCs) can be generated through directed differentiation or transdifferentiation. Employing two strategies, we generated induced pluripotent stem cell (iPSC)-HLCs and hiHeps from the same donor cell line. Both types of HLCs clustered distinctly from each other during gene expression profiling. In particular, differences existed in gene expression for phase II drug metabolism and lipid accumulation, underpinned by H3K27 acetylation status in iPSC-HLCs and hiHeps. While distinct phenotypes were achieved in vitro, both types of HLCs demonstrated similar phenotypes following transplantation into Fah-deficient mice. In conclusion, functional HLCs can be obtained from the same donor using two strategies. Global gene expression defined the differences between those populations in vitro. Importantly, both HLCs displayed partial but markedly improved hepatic function following transplantation in vivo, demonstrating plasticity and the potential for cell-based modeling in the dish and cell-based therapy in the future.
Source: Stem Cell Reports
Institutions: Chinese Academy of Sciences, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, State Key Laboratory of Cell Biology, Shanghai Institutes for Biological Sciences, Shanghai Institute of Materia Medica, East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, MRC Centre for Regenerative Medicine, University of Edinburgh, Shanghai Jiao Tong University, International Peace Maternity & Child Health Hospital, ShanghaiTech University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,190
Funders: Commonwealth Scientific and Industrial Research Organisation, National Natural Science Foundation of China, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Science and Technology Commission of Shanghai Municipality, University of Tsukuba, UK Regenerative Medicine Platform, Medical Research Council
OpenAlex ID: W2769935541