HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study
Year: 2017
Type: article
Abstract: The process, how lipids are removed from the circulation and transferred from high density lipoprotein (HDL) - a main carrier of cholesterol in the blood stream - to cells, is highly complex. HDL particles are captured from the blood stream by the scavenger receptor, class B, type I (SR-BI), the so-called HDL receptor. The details in subsequent lipid-transfer process, however, have not yet been completely understood. The transfer has been proposed to occur directly at the cell surface across an unstirred water layer, via a hydrophobic channel in the receptor, or after HDL endocytosis. The role of the target lipid membrane for the transfer process, however, has largely been overlooked. Here, we studied at the single molecule level how HDL particles interact with synthetic lipid membranes. Using (high-speed) atomic force microscopy and fluorescence correlation spectroscopy (FCS) we found out that, upon contact with the membrane, HDL becomes integrated into the lipid bilayer. Combined force and single molecule fluorescence microscopy allowed us to directly monitor the transfer process of fluorescently labelled amphiphilic lipid probe from HDL particles to the lipid bilayer upon contact.
Source: Scientific Reports
Institutions: Johannes Kepler University of Linz, TU Wien, Medical University of Vienna, Keysight Technologies (Austria), Queens College, CUNY, Arizona State University, University of Oxford, MRC Weatherall Institute of Molecular Medicine, MRC Human Immunology Unit, Max Planck Institute of Molecular Cell Biology and Genetics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,688
Funders: Wellcome Trust, University of Oxford, Österreichischen Akademie der Wissenschaften, Austrian Science Fund, Österreichische Forschungsförderungsgesellschaft, Bundesministerium für Wissenschaft und Forschung, Medical Research Council, Biotechnology and Biological Sciences Research Council, European Regional Development Fund
Awards: G1258001995, Physiological relevance of HDL uptake and resecretion, MICA: Nanoscopy Oxford (NanO): Novel Super-Resolution Imaging Applied to Biomedical Sciences, Micron Oxford: super-resolution imaging of cellular dynamics., G2205214540, G2509476059, Transfer of miRNA from single HDL particles to cells, Trafficking of HDL and its lipids in mouse models, G4900274166, G5013441748, G5285405813, G5290307715, Advanced super-resolution fluorescence STED microscopy of the cellular interior., G5637728360, G738990282, G984412895
OpenAlex ID: W2770123330