Impact of Virucide; Carrageenan in Suppression of Potato Virus Y in Potato Plants
Year: 2017
Type: article
Abstract: Background: Potato plants are usually subjected to numerous diseases caused by viruses. Potato virus Y belongs to a group of potyvirus that causes serious diseases to potato plants and is mainly responsible for the majority of losses caused in potato production. Objective: The present study aims to evaluate the potential effect of carrageenan (virucide) which was extracted from red algae (Acanthophora specifira) in an induction of systemic acquired immunity for bio-control of PVY infection in potato plants. Methods: Potato tubers (Solanium tuberoisum cv. spounta) were obtained. The virus isolate was obtained and maintained on Datura metel plant. Specimens of the red alga Acanthophora specifira were collected from El Shoaiba coast, the eastern part of Saudi Arabia; Red Sea. The tested material carrageenan was extracted from the selected seaweeds species as antiviral agents. The infectious sap was prepared and inoculated on the primary and free potato leaves. The tested plants were sub-classified into 5 groups; 2 healthy groups (control group and virucidetreated group), 3 PVY infected plants groups (one without virucide, PVY infected plants either before or after virucide). Reduction in the disease severity was recorded and inhibition in PVY infectivity as a result of periodical treatment of foliar potato plants with virucide (carrageenan) was assessed in pre and post-treatment groups. Results: PVY infection resulted in significant decrease in shoot length, leave an area and fresh and dry weight of shoots. Improvement of morphological characters of periodically-treated foliar with virucide (carrageenan) was observed. Chlorophyll and carotenoids contents were increased in PVY infected foliar potato plants treated with virucide (carrageenan). Biochemical markers as indicators for systemic acquired resistance were detected via significantly increased total proteins, phenol compounds, free proline (PO), polyphenol oxidase (PPO) and superoxide dismatase (SOD) enzyme activities. As well as expressed proteins of induced potato plants were determined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results indicated that newly expressed proteins were produced and a difference in number and density of bands in foliar treatment plants with virucide (carrageenan) were compared with healthy ones. Conclusion: It has been suggested that the induction of systemic acquired resistance (SAR) was successfully achieved; it also protected potato plants against PVY infection.
Author: Gomaa Hanaa H. A.
Institution: Suez Canal University
Language: English
Topic: Plant Virus Research Studies
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2771732096