A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle
Year: 2018
Type: article
Abstract: Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study how sarcomeres are built, we performed transcriptome sequencing of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, two clusters are strongly enriched for sarcomeric components. Temporal gene expression together with detailed morphological analysis enabled us to define two distinct phases of sarcomere development, which both require the transcriptional regulator Spalt major. During the sarcomere formation phase, 1.8 μm long immature sarcomeres assemble myofibrils that spontaneously contract. During the sarcomere maturation phase, these sarcomeres grow to their final 3.2 μm length and 1.5 μm diameter and acquire stretch-sensitivity. Interestingly, the final number of myofibrils per flight muscle fiber is determined at the onset of the first phase. Together, this defines a biphasic mode of sarcomere and myofibril morphogenesis – a new concept that may also apply to vertebrate muscle or heart development.
Source: eLife
DOI: 10.7554/elife.34058
Authors: Maria L Spletter, Christiane Barz, Assa Yeroslaviz, Xu Zhang, Sandra B Lemke, Adrien Bonnard, Erich Brunner, Giovanni Cardone, Konrad Basler, Bianca H Habermann, Frank Schnorrer
Institutions: Max Planck Institute of Biochemistry, Ludwig-Maximilians-Universität München, Centre National de la Recherche Scientifique, Foshan University, Aix-Marseille Université, Institut de Biologie du Développement Marseille, Inserm, Theories and Approaches of Genomic Complexity, University of Zurich
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,318
Funders: European Molecular Biology Organization, Alexander von Humboldt-Stiftung, Aix-Marseille Université, National Institute for Health and Care Research, Boehringer Ingelheim Fonds, Agence Nationale de la Recherche, Max-Planck-Gesellschaft, Centre National de la Recherche Scientifique, National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases, H2020 European Research Council
Awards: INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE, G1688594902, G2441619162, Dissection of the Salm Mediated Transcriptional Switch to Fibrillar Myogenesis, G4231765500, G4868159144, G572841159, Flux d'inFormation et organisation de la membrane, G7193312978, In vivo quantification and manipulation of mechanical forces across muscle attachment sites and sarcomeric proteins during muscle morphogenesis, Développment d'une infrastructure française distribuée coordonnée, G9000208451
OpenAlex ID: W2805464558