Year: 2003
Type: article
Abstract: A novel, simple, economical, and environmental friendly tunable immunosorbent-based immunoassay for sensitive and selective determination of atrazine is reported. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single chain Fv of an anti-atrazine antibody was synthesized biologically and purified by temperature-triggered phase transition. A competitive immunoassay based on the competition of atrazine-HRP and atrazine was established with IC50 and lower detection limit of 0.16 and 0.01 ppb, respectively. Excellent recoveries (mean values ranging between 92 % and 104 %) were demonstrated in simulated atrazine-contaminated water samples.
Authors: Wilfred Chen, Ashok Mulchandani
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2776115047